Vibrio anguillarum ile infekte edilmiş gökkuşağı alabalıklarında (Oncorhynchus mykiss) hematolojik incelemeler

Tez Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, Türkiye

Tez Danışmanı: Soner Altun

Tezin Onay Tarihi: 2010

Tezin Dili: İngilizce

Özet:

The aim of this study was to investigate haematological changes which caused vibriosis occured by experimentally infected with Vibrio anguillarum on rainbow trouts (Oncorhynchus mykiss). 100 rainbow trouts weighing 208.7±8.11 gr were used for this research. Vibrio anguillarum at a concentration of 100 bacteria/ml was injected by intraperitoneally (i.p.) amount 0.2 mL to 50 fishes and infected group was occurred. The blood samples of five fishes each infected and control groups were collected from caudal vein in 1, 2, 3, 4, 5, 7, 9, 11, 13 and 15 days after the infection by injector and examined in terms of erythrocyte, platelet and leukocyte counts, leukocyte types, haemoglobin, haematocrit, erythrocyte indices (mean cell volume (MCV), mean cell haemoglobin (MCH), mean cell haemoglobin concentrations (MCHC)), total plasma protein, albumin, and cortisol levels.

As a result of investigation, erythrocytes, leukocytes, haemoglobin, haematocrit, albumin and total plasma protein levels were decreased, however cortisol levels and platelet counts were increased in the infected groups. Neutrophil and monocyte cells were increased at the beginning of the experiment and were decreased in days following; on the other hand, lymphocytes were decreased at the beginning of the experiment and were increased in in days following depend on the disease process in the infected groups.

Reduction in the number of lymphocytes and increase number of neutrophils in the early days of the disease was revealed that the disease was acute clinical course.