Detection of Quorum Sensing System (Cell to Cell Communication) Using Marker Strains in Vibrio alginolyticus Strains and Determine Virulence under Master of this System

Filik N., KUBİLAY A.

Journal of the Hellenic Veterinary Medical Society, vol.73, no.4, pp.4945-4956, 2022 (SCI-Expanded, Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 73 Issue: 4
  • Publication Date: 2022
  • Doi Number: 10.12681/jhvms.28525
  • Journal Name: Journal of the Hellenic Veterinary Medical Society
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, Veterinary Science Database
  • Page Numbers: pp.4945-4956
  • Keywords: N-acyl homoserine lactones (AHLs), Quorum sensing, signal molecules, Vibrio alginolyticus, virulence factors
  • Isparta University of Applied Sciences Affiliated: Yes

Abstract

There are intercellular communication and social behaviors, including Quorum Sensing System, QS, (cell to cell communication) in bacteria. Aim of present study was to investigate production of QS signal molecules (N-acyl homoserine lactones, AHLs) and also determine virulence factors regulation of QS in V. alginolyticus. QS of V. alginolyticus was proved by cross validation assay via AHL using marker bacteria strains (Agrobacterium tumefaciens NT1 and Chromobacterium violaceum CV026). V. alginolyticus did not produced N-butanoyl homoserine lactone (BHL), while N-(3-okzododekanoyl)-L-homoserine lactone (OdDHL) production was detected in V. alginolyticus. The presence of virulence factors, such as biofilm, pigment, rhamnolipid, hemolysis, elastase, protease and amylase production investigated in V. alginolyticus as phenotypically.V. alginolyticus was found produce biofilm, pigment, amylase and elastase; however, it was not determined rhamnolipid, hemolysis and protease virulence.