Two crinivirus threatening greenhouse cucumber and melon plants in mediterranean region of Turkey: Cucurbit yellow stunting disorder virus (CYSDV), Cucurbit chlorotic yellows virus (CCYV)

Arslan S., ÇULAL KILIÇ H., Yardimci N.

Fresenius Environmental Bulletin, cilt.29, sa.9, ss.7980-7986, 2020 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 9
  • Basım Tarihi: 2020
  • Dergi Adı: Fresenius Environmental Bulletin
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.7980-7986
  • Anahtar Kelimeler: CCYV, Cucurbits, CYSDV, DAS-ELISA, Phylogenetic analysis
  • Isparta Uygulamalı Bilimler Üniversitesi Adresli: Evet

Özet

In 2015-2016 production season in Antalya, which is the most important region of greenhouse cultivation of vegetables in Turkey, severe yellowing symptoms which started especially from old leaves in cucumber and melon production areas were observed. 460 suspected leaf samples were tested with DAS-ELISA for CCYV (Cucurbit chlorotic yellows virus) and CYSDV(Cucurbit yellow stunting disorder virus) criniviruses. As a result of the DAS-ELISA tests, 46% of the samples have been found infected with CCYV and 38% with CYSDV. Mixed infections of two viruses were determined in 16% of the samples. The populations of Bemisia tabaci were densely observed on the plants that show yellowing symptoms. In the RT-PCR studies, DNA fragment that belongs to the CP and hsp70h gene regions of the isolates were obtained with CCYV and CYSDV specific primers. Partial base sequence analysis of CCYV and CYSDV isolates was performed. 91-95 % similarity between the CP gene regions of CCYV isolates obtained from different regions of the world with CCYV 22 isolate was determined. The isolate CYSDV54 was found to be similar in terms of hsp70h gene sequences between 91-100%) with CYSDV isolates obtained from different regions of the world in gene bank. Antalya CYSDV54 isolate and Antalya CCYV22 isolate were registered to the gene bank with accession numbers MK129537 and MK129538, respectively. This is the first report of CCYV infection of melon production areas in Turkey.