New rRNA primers for the detection of Vibrio anguillarum

Avsever M. L., Tunalıgil S., DİDİNEN B. I., METİN S.

Israeli Journal of Aquaculture - Bamidgeh, vol.67, 2015 (SCI-Expanded, Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 67
  • Publication Date: 2015
  • Journal Name: Israeli Journal of Aquaculture - Bamidgeh
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: PCR, Vibrio anguillarum
  • Isparta University of Applied Sciences Affiliated: Yes

Abstract

In this work, 51 V. anguillarum isolates (42/51 of O1 serotype, 9/51 of O2) obtained from cultivated marine fish by Avsever and Ün (2015) were used. These isolates were confirmed with universal primers specific to amiB genes. They were also detected successfully at a rate of 100% with a new primer couple designed according to 16S ribosomal RNA sequence. The new primers were found to have a high sensitivity and specificity but were liable to give a cross-reaction with Vibrio ordalii.