Serological and molecular characterization of the cucurbit aphid-borne yellows virus affecting cucurbits in Southern Turkey

Arslan S., Yardimci N., ÇULAL KILIÇ H.

Fresenius Environmental Bulletin, cilt.29, sa.9, ss.7239-7245, 2020 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 9
  • Basım Tarihi: 2020
  • Dergi Adı: Fresenius Environmental Bulletin
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.7239-7245
  • Anahtar Kelimeler: CABYV, Cucurbits, PCR, Phylogenetic, TAS-ELISA
  • Isparta Uygulamalı Bilimler Üniversitesi Adresli: Evet

Özet

Recently, severe yellowing virus symptoms were observed in cucurbit greenhouses in Antalya province, the major cucurbit-growing area in Turkey. In order to investigate the related virus, surveys were carried out in the greenhouses in the region, producing melon {Cucumis melo), cucumber (Cucumis sativus) and zucchini {Cucurbita pepo), during 2015 and 2016 vegetation period. Severe yellowing symptoms on older leaves of cucurbits and large populations of aphids {Aphis gossypii) on a diversity of cucurbit crops supported the hypothesis of a viral cause of the disease. A total of 460 {281 cucumber, 95 melon and 84 zucchini) suspected plant samples were taken. Virus identification using triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), followed by reverse transcription-polymerase chain reaction (RT-PCR) showed Cucurbit aphid-borne yellows virus (CABYV) infections on melon (30 samples), cucumber (117 samples) and zucchini (81 samples) plants. Partial coat protein (CP) was amplified by RT-PCR and sequenced from 5 isolates. One of the isolates (AKK10) was submitted to GenBank (GenBank accession number: MK129536). Phylogenetic analysis results showed that this isolate was similar with Egyptian and Italian isolates with a rate of 100%, whereas the least similarity (96%) was found with Indian and China isolates.