In vitro inhibition of polyphenol oxidase by some new diarylureas

DEMİR D., Gençer N., Arslan O., Genç H., Zengin M.

Journal of Enzyme Inhibition and Medicinal Chemistry, vol.27, no.1, pp.125-131, 2012 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 1
  • Publication Date: 2012
  • Doi Number: 10.3109/14756366.2011.580743
  • Journal Name: Journal of Enzyme Inhibition and Medicinal Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.125-131
  • Keywords: Diarylureas, Enzymatic browning, Inhibition, Polyphenoloxidase
  • Isparta University of Applied Sciences Affiliated: No

Abstract

A new series of N,N′-diarylureas (19) was synthesized. These compounds were investigated as inhibitors of polyphenol oxidase (PPO) which had been purified from banana by an affinity gel comprised of Sepharose 4B-l-tyrosine-p-amino benzoic acid. Ki values for (1), (2), (3), (5), (6), (7) and (8) were determined as 0.285, 17.97, 0.187, 0.108, 0.063, 0.044 and 0.047mM, respectively. Thus (2) was by far the most effective inhibitor. Interestingly, (4) and (9) behaved as an activator of PPO in this study. © 2012 Informa UK, Ltd.