PCR screening of barley genotypes in relation to cold hardiness

TAŞPINAR M. S., Sagsoz S., AĞAR G., TOSUN M., AKGÜN İ.

International Journal of Botany, vol.4, no.3, pp.315-320, 2008 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 4 Issue: 3
  • Publication Date: 2008
  • Doi Number: 10.3923/ijb.2008.315.320
  • Journal Name: International Journal of Botany
  • Journal Indexes: Scopus
  • Page Numbers: pp.315-320
  • Keywords: Barley, Cold-induced genes, Environmental stress, Field survival index
  • Isparta University of Applied Sciences Affiliated: Yes

Abstract

Several molecular methods were tested to distinguish 24 barley genotypes based on cold hardiness. We used a convenient, efficient and reliable PCR based method for barley to determine the cold tolerant and sensitive genotypes. Cold hardiness was strongly correlated with the existence of cold-induced genes. Significant differences were found based on Field Survival Index (FSI) of genotypes. Based on combination of FSI values and PCR detection pattern of cold-induced genes, PCR analysis can be used in plant breeding programs in order to shorten selection period at environmental stress studies and get exact results. © 2008 Asian Network for Scientific Information.