In vitro effects of some anabolic compounds on erythrocyte carbonic anhydrase i and II

Gençer N., Ergün A., DEMİR D.

Journal of Enzyme Inhibition and Medicinal Chemistry, cilt.27, sa.2, ss.208-210, 2012 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 2
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3109/14756366.2011.584535
  • Dergi Adı: Journal of Enzyme Inhibition and Medicinal Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.208-210
  • Anahtar Kelimeler: Anabolic compounds, Carbonic anhydrase, Inhibition
  • Isparta Uygulamalı Bilimler Üniversitesi Adresli: Hayır

Özet

The in vitro effects of the anabolic compounds, zeranol, 17 β-estradiol, diethylstilbestrol (DES), and trenbolone, on the activity of purified human carbonic anhydrase I and II were evaluated. In vitro CA enzyme activity was determined colorimetrically using the CO2 hydration method of Maren. IC 50 values of the compounds that caused inhibition were determined by means of activity percentage diagrams. The IC 50 concentrations of zeranol, 17 β-estradiol, DES and trenbolone on hCA I were 94, 55, 10, 898 M and for hCA II 89, 159, 439 and 101 μM, respectively. © 2012 Informa UK, Ltd.