Akademik Veri Yönetim Sistemi
Acta Horticulturae, cilt.1297, ss.667-674, 2020 (Scopus)
Almond shows self-incompatibility controlled by S-allele. The evaluation of an almond collection using a PCR based identification of self-compatibility and -incompatibility alleles may be beneficial for selecting pollinizers and designing crosses in almond breeding programs. In this study, self-incompatibilities were determined by PCR analyses in 42 almond genotypes, selected from Isparta region of Turkey as a result of a previous breeding study. Five pairs of primers that amplified different self-incompatibility S-alleles were used. PCR using combined primers AS1IIF and AmyC5R showed amplification of nine self-incompatibility alleles (S1, S2, S3, S5, S7, S9, S10, S11 and S13) and one self-compatibility allele (Sf). Among those, S1 and S2 alleles had the highest frequencies among the genotypes as compared to the other S-alleles. Also, the combination of CebasF and Amyc5R primers amplified only two S alleles (S5 and S10) in two genotypes. While S9 allele was detected in the genotype 129, only, each of the S8 and S23 alleles was determined in three genotypes. The results showed that the almond genotypes evaluated had both homozygous and heterozygous S-RNase alleles and 31 of the genotypes possessed self-incompatibility alleles. The identification of S-Alleles will be beneficial for designing crosses and for selecting progenies in breeding programs as well as for planning new commercial orchards in the future.