Phylogenetic analysis of some fungi species in West Kazakhstan based on nuclear ribosomal DNA ITS sequences

Sarsenova A. N., Sevİndİk E., Abievich A. S., Sofyalioğlu E., Esenomanovna D. T., EKEN C.

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, cilt.50, sa.3, 2022 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.15835/nbha50312783
  • Dergi Adı: Notulae Botanicae Horti Agrobotanici Cluj-Napoca
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aqualine, CAB Abstracts, Pollution Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Anahtar Kelimeler: Fungi, Its, Phylogenetic analysis, West kazakhstan
  • Isparta Uygulamalı Bilimler Üniversitesi Adresli: Hayır

Özet

In this study, phylogenetic analysis of some fungal species distributed in West Kazakhstan was performed based on ITS sequences. All of the mushroom samples were collected from different regions of West Kazakhstan and brought to the laboratory. Total genomic DNA was extracted using a GeneMark commercial kit. ITS1 and ITS4 primers were used for the amplification of the ITS region in PCR analyses. The resulting DNA sequences were then edited using BioEdit and FinchTV. For phylogenetic analysis used MEGA 6.0 program. As a result of the study, ITS sequences ranged from 532 to 715 nucleotides, while the divergence values of the sequences differed between 0.000 and 0.468. The maximum likelihood tree constructed using ITS sequences consists of two clades. According to the phylogenetic analysis results obtained using other fungal species, ITS results were found to be a good indicator for the differentiation of fungal genera.