Akademik Veri Yönetim Sistemi
Fresenius Environmental Bulletin, cilt.21, sa.3, ss.549-552, 2012 (SCI-Expanded, Scopus)
The effects of Imazethapyr, 2, 4-D dimethylamine salt, Glyphosate isopropylamine salt and Propamocarb HCl, which are commonly used in agricultural fields, have been investigated on human erythrocyte carbonic anhydrase isoenzymes (hCA-I, hCA-II) in vitro. Isoenzymes employed in the study were purified by using Sepharose-4B-Ltyrosine-sulphanylamide affinity gel and the purity was confirmed by SDS-PAGE. In vitro CA enzyme activity was determined colorimetrically using the CO 2 hydration method of Maren. All the pesticides evaluated inhibited the activity of isoenzymes to various degrees. IC 50 values of chemicals caused inhibition were determined by means of activity percentage-[I] diagrams. These values of Imazethapyr, 2, 4-D dimethylamine salt, Glyphosate isopropylamine salt and Propamocarb HCl, for CA-I and CA-II were 0.093 mM, 0.463 mM, 0.617 mM, 0.46 mM and 0.152 mM, 0.628 mM, 0.904 mM, 0.62 mM, respectively. Imazethapyr was the most effective inhibitors for CA-H isoenzyme. © by PSP.