24-Epibrassinolide and methyl jasmonate can encourage cell growth and the production of secondary metabolites in immobilized cells of Hyoscyamus niger
In Vitro Cellular and Developmental Biology - Plant, vol.58, no.5, pp.826-836, 2022 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 58 Issue: 5
- Publication Date: 2022
- Doi Number: 10.1007/s11627-022-10313-5
- Journal Name: In Vitro Cellular and Developmental Biology - Plant
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Veterinary Science Database
- Page Numbers: pp.826-836
- Keywords: 24-epibrassinolide, Hyoscyamus niger, Immobilized cell, Methyl jasmonate, Phenolics, Tropane alkaloids
- Isparta University of Applied Sciences Affiliated: Yes
Abstract
This study was carried out to determine the effects of 24-epibrassinolide (24-eBL) and methyl jasmonate (MeJA) on cell growth and accumulation of secondary metabolites in immobilized cells of Hyoscyamus niger. For this purpose, different combinations of 24-eBL (0.5, 1.0, and 2.0 mg l−1) and MeJA (1.0 mM) were applied to cells immobilized with calcium alginate. At the end of the 30-day applications, fresh and dry cell weights, cell growth index, tropane alkaloids (hyoscyamine and scopolamine), phenolic (gallic acid, catechin, chlorogenic acid, caffeic acid, epicatechin, vanillin, p-coumaric acid, o-coumaric acid, ferulic acid, rutin, cinnamic acid, rosmarinic acid, and quercetin), and total phenolic content were determined. According to the results, 2.0 mg l−1 24-eBL for cell growth, 0.5 mg l−1 24-eBL for scopolamine and hyoscyamine, and all 24-eBL combinations with 1.0 mM MeJA for phenolic compounds were selected as the most effective applications. In conclusion, it was determined that the application of 24-eBL and MeJA may be successfully used to increase the production of secondary metabolites in immobilized cell cultures of H. niger when used in appropriate concentrations and combinations. Graphical abstract: [Figure not available: see fulltext.].