Inflammation-mediated fetal injury by maternal granulocyte-colony stimulating factor and high-dose intra-amniotic endotoxin in the caprine model Maternal granülosit koloni stimülan faktör ve yüksek doz intra-amniyotik endotoksin ile enflamasyon aracılı fetal beyin hasarı keçi modeli

SEZİK M., Köker A., Özmen Ö., HALIGÜR M., KAŞIKCI D., AYDOĞAN A., ...Daha Fazla

Turkish Journal of Obstetrics and Gynecology, cilt.16, sa.1, ss.41-49, 2019 (Scopus, TRDizin) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 1
  • Basım Tarihi: 2019
  • Doi Numarası: 10.4274/tjod.tjod.galenos.2019.92300
  • Dergi Adı: Turkish Journal of Obstetrics and Gynecology
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.41-49
  • Anahtar Kelimeler: Animal model, Cerebral palsy, Chorioamnionitis, Endotoxins, Inflammation
  • Isparta Uygulamalı Bilimler Üniversitesi Adresli: Evet

Özet

Objective: To define a novel experimental model with maternal intravenous (i.v.) granulocyte-colony stimulating factor (G-CSF) followed by a single-and high-dose of 20 mg intra-amniotic (IA) endotoxin to induce fetal brain injury in the preterm fetal goat. Materials and Methods: Pregnant goats (n=4) were given 50 microg/day G-CSF into the maternal jugular vein through gestational days 110-115 (term, 150 days). At gestational day 115, 20 mg of IA endotoxin was administered. Following preterm delivery at day 120 by cesarean section umbilical cord, fetal lung and brain tissues were harvested for histopathology, immunohistochemistry, and electron microscopy. Inflammatory markers were evaluated in the amniotic fluid and fetal plasma. Results: Necrotizing funisitis with abundant leukocyte infiltration and fetal brain injury was induced in all the fetuses in the experimental group. Conclusion: Maternal i.v. G-CSF for 5 days followed by 20 mg of IA endotoxin is a feasible caprine model to exacerbate intrauterine inflammation.