Akademik Veri Yönetim Sistemi
Biologia Nyssana, cilt.15, sa.2, 2024 (Scopus)
In this study, phylogenetic analyses were performed using chloroplast rbcL sequences from Ajuga chamaepitys, Thymbra capitata, Lamium moschatum, Marrubium vulgare, Stachys cretica, Teucrium polium, and Vitex agnus-castus species distributed in Aydın province, Türkiye. After isolating DNA from the samples, PCR amplification was carried out using the rbcLaF and rbcLaR primers. Both forward and reverse sequences were edited using BioEdit 7.2.3, FinchTV 1.4.0, and Sequencher 5.4.6 software. The corrected sequences were converted to protein sequences, and phylogenetic analyses were conducted using MEGA 6.0 software. The rbcL proteins have been motif analysed and their 3D structure revealed. As a result, the phylogenetic tree constructed with sequences obtained from National Center for Biotechnology Information (NCBI) showed that Lamium and Marrubium species formed a clade, while Sideritis and Stachys species formed another one. Additionally, the species of Thymbra, Vitex, Ajuga, and Teucrium each formed distinct groups. In the analyses involving only these seven species, the genetic distance matrix revealed that the closest species were Ajuga chamaepitys and Vitex agnus-castus (0.009), while the most distantly related species were Teucrium polium and Thymbra capitata (0.043). Also, the nucleotide diversity was calculated to be π = 0.025278. Overall, the rbcL sequence results were instrumental in elucidating the phylogenetic relationships within the Lamiaceae family.