Micropropagation of Cupressus sempervirens L. and Chamaecyparis lawsoniana (A. MURR.) PAR.

Spanos K., Pirrie A., WOODWARD S.

Silvae Genetica, vol.46, no.5, pp.291-295, 1997 (SCI-Expanded, Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 46 Issue: 5
  • Publication Date: 1997
  • Journal Name: Silvae Genetica
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.291-295
  • Keywords: Chamaecyparis lawsoniana, Cupressus sempervirens, Micropropagation, Seiridium cardinale
  • Isparta University of Applied Sciences Affiliated: Yes

Abstract

Shoots from 18-month-old seedlings of Cupressus sempervirens and Chamaecyparis lawsoniana were established in vitro on modified MURASHIGE and SKOOG medium. Proliferation of axillary shoots occurred without addition of benzyladenine, although a significant increase in numbers of shoots resulted on addition of 0.001 mg.l-1 to 1.0 mg.1-1 benzyladenine. Following conditioning on a growth regulator-free medium for 28 days, 95% of C. sempervirens shoots rooted on 1/2 strength medium containing 1% sucrose and 0.5 mg.1-1 indole butyric acid. Similar levels of rooting were recorded with Chamaecyparis lawsoniana on the same medium containing 1 mg.l-1 indole butyric acid. C. sempervirens shoots were also rooted in non-sterile conditions. Over 80% of rooted shoots of C. sempervirens were weaned into glasshouse conditions. The significance of these results in relation to propagation of cypress clones resistant to Seiridium canker is discussed.