Bulletin of Insectology, cilt.76, sa.1, ss.133-139, 2023 (SCI-Expanded, Scopus)
The present study aimed to determine the damage level of biopesticides with different contents in bumblebees according to two different exposure scenarios: residual contact and spray application. Bombus terrestris (L.) (Hymenoptera Apidae) workers were exposed to Nibortem (1.5% Lecanicillium (Verticillium) lecanii strain V1-1), Nostalgist (1.5% Beauveria bassiana strain Bb-1), Priority (1.5% Isaria (Paecilomyces) fumosoroseus strain PFs-1), Nimbecidine (0.3 g/L Azadirachtin) and Nematac (Steinernema feltiae) at three different doses [1/1, 1/10 and 1/100], with both application methods. In the spray application, the suspension of each biopesticide was sprayed for 20 seconds (0.5 ml) to each box containing 10 workers. In the residual contact application, suspensions were sprayed into empty plastic boxes with the same method and then workers were transferred into these boxes and they remained in the treated boxes for 15 minutes only. The observations were carried out for 14 days in all treatments. Whereas the mean number of dead workers were significantly higher for Nibortem, Nimbecidine, Nostalgist, and 1/1 dose of Nematac in residual contact application, this value was found statistically different for Nibortem, Nimbecidine, Nostalgist, Priority, and 1/1 dose of Nematac in spray application, than control group (P ˂ 0.05). The mean number of dead workers in 1/10 and 1/100 doses of Nematac were not significantly different from the control in both application methods. There was no significant difference between both application methods in terms of death values for the control, Nostalgist, 1/1 dose of Nematac.