Akademik Veri Yönetim Sistemi
Research Journal of Biotechnology, cilt.16, sa.4, ss.75-80, 2021 (ESCI, Scopus)
Reactive oxygen species (ROS) increase in plants due to abiotic and biotic stresses. ROS interferes with intracellular substances and cell membrane to damaged plants. Plants have enzymatic and non-enzymatic antioxidant systems to protect themselves against ROS. Glutathione reductase (GR) and glutathione s-transferase (GST) are two of the antioxidant enzymes. The sequence information of these enzymes, which are extremely important for coping with stress, has not been investigated in many plants including strawberries. In this study, GR and GST antioxidant enzyme genes were isolated and characterized from Kabarla strawberry variety. For this purpose, degenerated primers were designed and partial cDNAs were amplified by PCR, and then 3’ and 5' RACE analysis were performed to obtain their full length nucleotide sequences. According to the sequence results, the GR and GST genes were composed of 2091 and 1007 nucleotides respectively and were found to have open reading frames of 557 and 287 amino acids. In addition, the isolated GR gene has shown high similarity to the genes isolated from Pisum sativum, Medicago truncatula and Cicer arietinum; the GST gene demonstrated high sequence similarity to the genes obtained from Arabidopsis thaliana, Zea mays and Prosopis juliflora.